In this study, species of the genus Mistshenkoana Gorochov, 1990 from China are studied based on molecular marker and morphological characteristics. Two new species, Mistshenkoana tianya He & Wei n. sp. and M. melanocephala He & Wei n. sp., are described. M. nhachangi Gorochov, 2007 is reported from China for the first time. Female genitalia of this genus are studied for the first time, and described here for two species, M. gouriatus Zheng, Xin, Xie & Ma, 2021 and M. kongtumensis Gorochov, 1990. We sequenced the fragments of the mitochondrial cytochrome-c oxidase subunit I gene (COI, 658 bp) from five Mistshenkoana species and provide the Genbank number. The molecular results support morphological classification.