Lasiodiplodia pseudotheobromae, a species recently segregated from L. theobromae, is reported from southern China for the first time. The identification was based on morphological features and comparison of DNA sequence data from nuc-ITS and partial tef-1α gene. Acacia confusa, Albizia falcataria, Eucalyptus sp., Mangifera sylvatica, and Paulownia fortunei are reported as new hosts for this species. A key to the Lasiodiplodia spp. mainly based on the characteristics of conidia and paraphyses is provided.