Echinosphaeria macrospora is a novel endophyte isolated from stems of Centella asiatica (Apiaceae/Umbelliferae) with its novel, hyphomycetous anamorph, Vermiculariopsiella endophytica. The fungus first produced the conidial state, followed by development of its teleomorph after 4 weeks of incubation. Echinosphaeria macrospora differs from the type of this monotypic genus, E. canescens, in having wider asci and larger ascospores. This is the first report of a sexual state amongst the species of Vermiculariopsiella and the third asexual stage of Echinosphaeria.