Coniophora bimacrospora, sp. nov. is described from the Ethiopian highlands. The taxon is mainly characterized by an olive green hymenial surface, bi-spored basidia, and large, averaging 20 × 15 μm, ellipsoid to broadly ovoid, brownish, cyanophilous, and dextrinoid basidiospores. It is compared to some other Coniophora reported from Africa. Coniophora puteana, C. olivacea, Serpula similis, and Gyrodontium sacchari are also reported from the Ethiopian highlands. The phylogenetic relationships between S. similis, G. sacchari, and other Coniophoraceae are briefly discussed.