Collembolispora barbata gen. et sp. nov. is described from a mountain freshwater stream in Portugal. Descriptions from pure cultures are provided for the following rare species: Flagellospora curta, Geniculospora grandis, Heliscus submersus, Tetracladium palmatum, Tricladiopsis flagelliformis and Tricladium terrestre. Conidia of Flabellospora amphibia and Titaeella capnophila are reported from stream foam.