Gliocladiopsis guangdongensis sp. nov. is introduced on the basis of both morphological and on multilocus phylogenetic analysis (β-tubulin, histone H3, internal transcribed spacer region and translation elongation factor 1-α). The species is compared to other morphologically similar and phylogenetically closely related taxa. Species of Gliocladiopsis are mostly known from soil and terrestrial plants whereas this new species is the first isolation from decaying wood from freshwater habitat.