The second contribution on the bryophytes of the Reserva Biológica San Francisco, Zamora-Chinchipe province, Southern Ecuador, adds 194 liverworts and mosses to the local bryophyte florula. Two species, Fuscocephaloziopsis subintegra Gradst. & Vána and Cyclodictyon provectum W.R. Buck are described as new to science; the latter species is illustrated with line drawings and SEM photographs. Twenty-five species (21 liverworts and 4 mosses) are first records for Ecuador. The additional records bring the local bryophyte florula to 499 bryophytes (313 liverworts; 183 mosses; 3 hornworts), the highest species number ever recorded from a relatively small area (1,000 ha) in the tropics.