Two new species of the genus Diplasiolejeunea from the Andes of southern Ecuador, D. erostrata and D. grandirostrata, are described and figured. A key to and a synopsis of the Andean species of Diplasiolejeunea are presented. Diplasiolejeunea caribea subsp. latetruncata, D. caribea subsp. microstipulata, D. caribea subsp. subcaribea, D. arietina, D. caricae, D. crassidentata, and D. reyesiana are considered to be conspecific with D. caribea.