A new species of the family Theraphosidae, Holothele sulfurensis n. sp., is described from the Soufrière area in Guadeloupe (FWI). The distinctive taxonomic characters are: scopulate metatarsi IV, divided scopula on tarsi I, straight fovea, and simple, long and digitiform spermathecae. Its biology, compared with those of the other species of the recently revised genus, allows some remarks on the distribution of the genus Holothele Karsch, 1879 in the West Indies.
Arachnida, Araneae, Mygalomorphae, Theraphosidae, Holothele, French West Indies, Guadeloupe, distribution, new species.