Sri Lankan hornworts remain the least studied group of bryophytes. So far, three families (Anthocerotaceae, Notothyladaceae, Dendrocerotaceae) and 4 genera (Anthoceros, Folioceros, Phaeoceros and Dendroceros) have been recorded within the country. During the present study, hornworts were freshly collected from different geographical localities within the country and identification was done using light and stereo-microscopy and SEM spore morphological observations. Based on this study, Notothylas javanica (Sande Lac.) Gottsche (Notothyladaceae) and Megaceros flagellaris (Mitt.) Steph. (Dendrocerotaceae) are reported for the first time in Sri Lanka. The species are described and illustrated with notes on their ecology and distribution.