The bryophyte flora of Trento has been studied based on an extensive sampling. To standardize the harvesting, the city has been divided into 5 zones and 12 habitats, depending on the human impact, the substratum nature and moisture state. At least 10 samples of 20 × 20 cm have been collected in all available habitats in each zone. As a result, 136 taxa have been recorded, 12 of which were liverworts and 124 were mosses. This is the richest Italian town to date and one of the most diverse, showing an important number of new regional and national records and some remarkable threatened species. The best represented families are 6 (Pottiaceae, Brachytheciaceae, Grimmiaceae, Amblystegiaceae, Orthotrichaceae, Bryaceae and Hypnaceae) and those with only one species are 21 (comprising mosses and liverworts). Some of the species found in Trento are frequent in urban environments, but most of them are uncommon in such environments. This outstanding bryophyte flora can be due to the climatic conditions of Trento and to the presence of numerous microhabitats that are suitable for these cryptogams.