This study revelead the presence of several euglenophyte taxa (Euglenophyta) in Lake Chignahupan, in the Upper Lerma Basin (Mexico). A total of 21 taxa were identified, 10 belonging to the genus Phacus, 5 to Lepocinclis, 2 to Euglena, 2 to Monomorphina, one to Cryptoglena and one to Trachelomonas. The species Crytoglena skujae, Euglena caudata, E. limnophila, Lepocinclis ovum var. dimidio-minor, L. salina, L steinii, Monomorphina aenigmatica, Phacus caudatus, P. ichthydion, P. inflexus, P. orbicularis, P. polytrophos and P. raciborskii were recorded for the first time in Mexico. All observed taxa are described briefly and illustrated by microphotographs or line drawings.