An optimized protocol of single spore isolation for fungi

Ke ZHANG, Yuan-Ying SU & Lei CAI

en Cryptogamie, Mycologie 34 (4) - Pages 349-356

Published on 27 December 2013

Pure culture is important for taxonomic, physiological and various application studies of fungi. Many protocols for obtaining pure cultures have been reported, of which single spore isolation was used most frequently in mycological studies. However, previously reported methods were relatively complicated and time consuming. In this paper, an optimized protocol of single spore isolation is introduced. Compared to previous protocols, this protocol is favorable in terms of its lower contamination rate, is less time consuming, and requires only simplified procedures with common and easily accessible apparatus.

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