An annotated account of Hyphomycetes from Mil Cumbres Conservation Area, Pinar del Río province,Western Cuba, is given. Eighty-nine species belonging to 68 genera were compiled from short-term mycological field work carried out from 1998 to 2000, mycological literature and IES Mycology Department Data Bases. Dictyochaeta assamica, Menisporopsis novae-zelandiae and Ramoconidiifera verrucosa are recorded for the first time from Cuba. A new combination Solicorynespora garciniae (Peck) Delgado & J. Mena is proposed. Comments about the taxonomy, ecology and geographic distribution of interesting species are included.