Tityus oteroi n.sp. (Scorpiones, Buthidae) is described on the basis of five specimens (one male and four females), collected in the region of Darién in the north of Colombia. With the description of Tityus oteroi, the number of species of the genus Tityus known to be distributed in Colombia is raised to twenty. Some comments concerning the taxonomic position of the new species are added. A check list of Columbian species of Tityus as well as keys for their determination are also included.
Scorpiones, Buthidae, Tityus oteroi n.sp., Darién, Colombia, new species, check list, taxonomic key.