Ostreopsis cf. ovata in the French Mediterranean coast: molecular characterisation and toxin profile

Véronique SECHET, Manoella SIBAT, Nicolas CHOMÉRAT, Elisabeth NÉZAN, Hubert GROSSEL, Jean-Brieuc LEHEBEL-PERON, Thierry JAUFFRAIS, Nicolas GANZIN, Françoise MARCO-MIRALLES, Rodolphe LEMÉE & Zouher AMZIL

en Cryptogamie, Algologie 33 (2) - Pages 89-98

Published on 25 May 2012

The presence of dinoflagellates of the genus Ostreopsis along Mediterranean coasts was first observed in 1972, in the bay of Villefranche-sur-Mer. However, over the past ten years, harmful events related to this benthic dinoflagellate have been reported in Italian, Spanish, Greek, French, Tunisian and Algerian coastal areas. In France, during a hot period in August 2006, cases of dermatitis and respiratory problems were registered in Marseille area. At that time, a link to the proliferation of Ostreopsis was highlighted for the first time in that area. A specific monitoring was designed and implemented in the summer 2007.
Two strains of Ostreopsis cf. ovata, collected in 2008 from Villefranche-sur-Mer and Morgiret coastal waters and grown in culture, were identified by molecular analysis and studied to characterise their growth and toxin profile. Liquid chromatography-mass spectrometry (LC-MS/MS) indicated that both strains produced ovatoxin-a (OVTX-a) as the major component (ca. 90), and traces of palytoxin (PLTX). Toxin content was determined at the end of the exponential growth phase with highest concentration of 55 pg.cell-1 of OVTX-a and 2.5 pg.cell-1 of PLTX.

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